ISSN NO:2394-2401 (Online)

ISSN NO:2394-384X (Print)





International Journal of Sciences and Applied Research (IJSAR) is a peer reviewed, open access International Scientific Journal dedicated for rapid publication of high quality original research articles as well as review articles in major areas of basic and applied sciences. International Journal of Sciences and Applied Research is published monthly. IJSAR with an internationally recognized editorial board emphasizes on producing high quality research content in a timely manner.

We intend to build mutually beneficial and long-lasting relationships with our authors and provide them full support throughout the publishing and the post-publishing processes.

Tittle : Comparison of microscopic examination and PCR in the diagnosis  of Trichomoniasis  in  patients  attending  the  sexually transmitted  disease  and acquired  immune  deficiency  syndrome  control  program in  Kandy, Sri Lanka

Author(s) : H. M. S. P. Herath, W. M. D. R. Iddawela*, W. D. S. J. Wickramasinghe, G. C. Pathirana

Abstract      Download PDF


Trichomoniasis is a sexually transmitted disease (STD) in humans.  It is caused by a motile pathogenic protozoan, Trichomonas vaginalis. Clinical diagnosis of trichomoniasis is not reliable due to nonspecific clinical presentation, which overlaps with the clinical features of other sexually transmitted diseases. Therefore, confirmation of suspected clinical cases by laboratory tests is essential. The diagnosis is usually based on wet mount. The sensitivity of this method is lower than polymerase chain reaction (PCR). Therefore, in the present study we evaluated PCR as a diagnostic tool for diagnosis of trichomoniasis in Sri Lanka for the first time.
Female patients attending the sexually transmitted disease and acquired immune deficiency syndrome (STD/AIDS) control program in Kandy were included in the study. Patients’ demographic data, clinical status and three vaginal swabs were obtained from the each patient separately. Two vaginal swabs were used for wet mount and permanent staining respectively. Other vaginal swab was used to isolate genomic DNA. PCRs were performed using the genus and species specific primers.
151 patients  were  studied  during  the  period  from  May  2015  to November 2015. Out of these, majority of patients (87/151) were aged between 15 to 35 years. 97 patients were clinically symptomatic. Of 151 samples, three were positive for trichomoniasis by direct smears. 8 samples were positive for PCR confirming the etiological diagnosis as T. vaginalis. 
The findings of the study suggest that PCR can be used to diagnose clinically suspected trichomoniasis patients in STD clinics in Sri Lanka.

Printed and Published by: SOFTLINK Publication Network, INDIA
All rights reserved ©

Disclaimer: Articles on IJSAR have been previewed and authenticated by the Authors before sending the publication for print. The Journal,
Editor and the editorial board are not entitled or liable to either justify or responsible for inaccurate and misleading data if any. It is the sole responsibility of the Author concerned.