Background: Staff nurses are at increased risk of occupational acquisition of HIV, HBV, and other viral infections due to accidental exposure of infected blood and body fluids. Needle stick injuries not only potentiate health consequences but also cause emotional distress, which results in missed work days which in turn directly affects the heath care services and resources.

Objective: To estimate the prevalence of needle stick injury among staff nurses in a tertiary care hospital and to study factors associated with needle stick injury.

Methodology: This  cross  sectional  study is conducted among staff  nurses in a tertiary care hospital for a period of 1 month.100 staff nurses were selected from sampling frame of 350 by Systematic random sampling

Results:  About 62% of staff nurses had needle stick injury ever during their work period in various hospitals. Most of the injuries 53(86%) occurred while injecting drugs. Nearly 38(62%) of needle stick injuries occurred during night shift. 47(76%) of needle stick injuries have occurred within one year of their experience.