Abstract
The water buffalo holds tremendous potential in the livestock sector in Asian and Mediterranean countries due to their diversified advantages. Faster multiplication of superior genotypes and the conservation of endangered buffalo breeds are crying need for the buffalo scientists. Recent advances in assisted reproductive biotechnologies, including male and female assisted technologies, offer enormous opportunities to not only improve productivity, but also to use buffaloes to produce novel products for applications to human health and nutrition. In this paper, an account of various aspects related to buffalo reproduction has been discussed. This paper included an overview of the most recent developments of reproductive biotechnologies, new strategies of reproductive management for the improvement of genetic gain and the application of newly developed reproductive technologies, such as in vitro embryo production, embryo and sperm sexing and cloning of buffaloes.