Objective: To investigate the effect of microRNA(miR)⁃221 in proliferation of tongue squamous cell carcinoma(TSCC) cells in vitro and in vivo.
Methods: The UM1 cell line was transfected with miR⁃221 inhibitor, and qRT⁃PCR analysis was used to detect the expression level of miR⁃221 in UM1 cell line after transfection. MTS assay was performed to analyze the effect of miR-221 on proliferation after transfection. Western blot analysis was used to demonstrate the metastasis associated proteins after transfection. Tumorigenesis model in nude mice was performed to analyze the effect of miR-221 on proliferation of UM1 cell line after transfection in vivo. Data were analyzed using the Statistical Package for the Social Science (SPSS), Version 19.0. For all statistical analyses, P<0.05 was considered statistically significant.
Results: Ectopic transfection of the miR-221 inhibitor to the UM1 cell led to decrease inmiR-221 expression (P<0.01); after transfection, the proliferation rate of UM1 cell was reduced (P<0.05); the expression level of E-Cadherin in cells was increased, while that of the MMP2, MMP9, Vimentin were decreased. The results of the subcutaneous xenograft model in nude mice showed that low-expression of miR-221 led to decrease in tumorigenesis ability (P< 0.01). Immunohistochemical staining showed that E-Cadherin was increased, and MMP2, MMP9, Vimentin were decreased.
Conclusion: miR-221 gene silencing could suppress the proliferation of TSCC in vitro and in vivo.

This work was aimed at evaluating the heavy metal, microbial and helminthic quality of edible clay (bentonite Samples were procured at different markets in Port Harcourt, bentonite contains some trace elements with concentrations such as zinc (0.152mg/kg), copper (<0.001-1.15mg/kg), lead (0.005mg/kg), cadmium (<0.005mg/kg) and nickel (0.02-12.7mg/kg). The THB counts ranged from 3.5 x 106 to 5.5 x 106, while Total Fungal culture ranged from 2.9 x 103 to 3.3 x 103.  The bacterial species isolated include Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumonia, Serratiasp and Aeromonas sp. While. Fungi cultures include: Aspergillusniger Saccharomyces sp, Aspergillusoryzae, Fuscarium sp. Cladosporiumsp, Penicilliumsp and Aspergillussp Edible clay has no antimicrobial effect against tested isolates n S.aureus, E coli,K. puemonia, P. aeruginosa and  C .albican using well agar and disk diffusion method.. Edible clay was also observed for the helminthes, the eggs of Ascarislumbricoides and Ancylostomaduodenale found to be associated with the clay. Benthonite harbours pathogenic microorganisms and parasites. probably because of the source and handling or packaging.

This study was aimed to identify phytochemical screening of the medically important species; Hydnora africana fruit extracts and to evaluate its potential antimicrobial and antioxidant activities. The samples were collected from Karal village west Nyala, South Darfur State Sudan. The extraction of crude was carried out at Standards and Metrology Laboratories in (El-Obeid) then analyzed at National Research Center in Khartoum in December 2020. Phytochemical screening of secondary metabolites of the plant crude extracts (methanol, acetone, petroleum ether, chloroform and distilled water) were carried out. The results revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids, coumarins, quinones, cardiac glycosides, xanthoproteins, glycosides, steroids, phenols, resins and carboxylic acid in different concentrations. The antioxidant activity of the extract of Hydnora Africana exhibited A maximum DPPH scavenging activity of acetone extract was 88% whereas for the standard propyl gallate was (82±0.01). The antimicrobial activity of the extracts was evaluted using diffusion assay against two Gram positive bacteria: Staphylococcus aureus, Bacillus subtilis and two Gram negative bacteria: Eschericha coli, pseudomonas aeruginosa and fungi candida albicans, the methanolic extract was active against fungi candida albicansat concentration 100mg/ml. The methanol and acetone extracts were active but the petroleum ether extract was partially active against four types of bacteria, but the chloroform and distilled water extracts were inactive against four types of bacteria and fungi and no distinct inhibition zones at Concentration 100mg/ml. This study Recommend further antimicrobial activity test against others types of bacteria and fungi and use modern analytical methods of bioactive components in the plant.

The effect of Industrial activities on the concentration of anionic contaminants in corrosion products of mild steel buried in clay soils was assessed, and the presence of Cl-, SO42-, HCO3-, and CO32- ions increased with increase in concentration of the corrodents, with SO42- and CO32- ions having the highest concentrations as 39.345 and 34.121ppm, respectively and it was observed that where the anodic reaction products build up, a limited amount of Cl-, SO42-, CO32- or HCO3- ions also move up into the formed oxide or hydroxide films, more so, the presence of CO32- and HCO3- ions in the corrosion products stifles the corrosion process.

Jungle Jalebi (Pithecellobium dulce) is an underutilised traditional plant found all over of the India. By the finding of different studies, it can be concluded that it is a good source of protein (approximately 12g/100g on dry weight basis) and ascorbic acid (approximately 138mg/100g on fresh weight basis).  The presence of several bioactive components makes it an easily available cost-effective nutraceutical treasure. However, the scope of animal and human supplementation trials along with development of value-added products is still needed to fully reap nutritive and economic potential of Jungle Jalebi.

Stigma maydis also known as corn silk is a yellowish thread-like strand of female flower of maize belonging to Graminae family. Phytochemical constituents of corn silk of maize plant grown in a clay loam soil constitutes alkaloids, tannins, proteins, flavonoids, cyanogenic glucoside, terpenoid and saponins. The proximate analysis of corn silk showed that it has a moisture content of 86.70%, a protein content of 2.06%, an ash content of 1.20%, a lipid content of 0.20%, a level of carbohydrate of 9.31% and 0.53% of crude fibre. Potential use of corn silk is related to its properties and mechanism of action of its bioactive constituents such as flavonoids and terpenoids. This study indicates that corn silk possesses antihypertensive, antioxidant, anticancer, antidepressant, kaliuretic, neuroprotective properties. However, the phytochemical constituents and proximate chemical composition were suspected to be reduced as a result of high bulk density of the soil and ponding which may have delayed early shoot emergence, root formation, and stunts overall plant performance.

Red Book uplifted the  logical  approach  in Astrology. During the course of study, the  inferences  drawn  from  scientific  evaluation  of  several  horoscopes,  motivated  to  reformulate  some basic interesting  facts and propose  the  hypothesis  that  could streamline and help  in  better  understanding, correlating  and  systematic  precise  analysis  of  the  horoscope.  Firstly, it was  observed  that the  lordship  for  the  Zodiac sign  needs to be reframed. Secondly, the effect of one planet on other and its effect on neighboring houses in horoscope needs  amendment.  Thirdly,  it was established that Navmansh  chart (D9)  in  addition  to  Lagan  chart   determines  and  governs  the  complete  life  span  of  an  individual. The life of the native depends on lagan chart till thirty forth year while Navmansh chart rules the life from thirty fifth year and thereafter.

Laparoscopic cholecystectomy is the gold standard procedure for cholelithiasis. Pneumoperitoneum for laparoscopic cholecystectomy is most often created by insufflating carbon dioxide gas into the peritoneal cavity and then holding it at constant pressure till the end of surgery. Standard pressure pneumoperitoneum, employing a pressure range of 12-14 mm Hg, over prolonged periods has been associated with intra and postoperative adverse effects. An emerging trend has been the use of low-pressure pneumoperitoneum in the range of 7-10 mm Hg in an attempt to lower the impact of pneumoperitoneum on the human physiology while providing adequate working space. Our study proposes to compare the effects of low-pressure pneumoperitoneum with the use of standard pressure pneumoperitoneum with special reference to study   frequency and intensity of postoperative pain.

The paper designed to raise the sole of one side of the foot to indirectly cause body imbalance. detecting the surface electromyographic activities(SEMG) of sternocleidomastoid muscle (SCM), trapezius muscle (TR), temporal muscle(TA) and masseter muscle (MM) in mandibularpostural posture (MPP). So as to analyzed the influence of plantar afferent signal changes on the above muscle functions, and elaborated the correlation between body posture and maxillofacial and neck muscle functions. Eleven asymptomatic subjects (4 males and 7 females, ages 20-30 years) were included in this study. Disturbing the balance of body posture by lifting the one side of foot with hard boards in MPP. Recorded the SEMG of TA, MM, SCM, TR by using COMETA surface electromyography in the condition of 0.5cm, 1.0cm and 1.5cm of the planthole padded up on one side and without the planthole padded up. Analysing the changes of the average SEMG of TA, MM, SCM and TR under different plantar pad heights in MPP. In the unpadded condition, the SEMG of SCM was the most obvious, followed by TA and TR, and the SEMG of MM was the weakest. The average SEMG of left SCM and TR were significantly higher than those of right SCM and TR (P<0.05). Under the condition of respectively raising the left and right plantar, the average SEMG of the padded and non-padded TR increased gradually with the increase of the foot height step by step, which was significantly higher than that of the ipsilateral TR in the unpadded condition (P<0.05). The average SEMG of the pad side increased with the increase of the foot height (P<0.05), but not obvious on the unpad side (P>0.05). The average SEMG of the left MM decreased with the increase of the right planthar height (P<0.05), while there was no statistical difference in the average SEMG of the high side and the non-high side compared with the same side under the other high conditions (P>0.05). And the average SEMG of TA was also not significantly affected by the height of the plantar pad (P>0.05). Based on these findings, it can concluded that the body posture imbalance caused by unilateral plantar cushion can change the SEMG of sternocleidomastoid muscle, trapezius muscle, temporalis muscle and masseter muscle in mandibular posture. The sternocleidomastoid muscle and trapezius muscle have strong response to plantar cushion stimulation, so as to improve the EMG activity intensity in mandibular posture and maintain the normal position of the mandible. This study reveals that the change of body posture caused by the change of plantar afferent signal can regulate and stabilize the mandibular position through the ascending muscle chain.

Introduction of SCADA system in Generation, Transmission and Distribution sectors of our electricity industry has changed the traditional way of doing our routine work in the industries. SCADA monitors the status of various equipment in the substations and sends control signals to the remote control equipment on a continuous basis. It gets the historical data of the substation and generates the alarms in the event of electrical accidents or faults. Most substations now utilize the SCAD system to monitor measurements, of relevant parameters for correction and regulation of end devices. Certain measurements, supervision, control, operation and protection functions are performed using the technology.. The problem of not controlling power substation properly by the conventional approach has made some areas not to have power supply as at the time they are meant to have it. That is the reason SCADA control of power substation applying programmable Logic Controllers is now adopted by power systems Engineers. It has improved the overall efficiency of operating power system components. The design is done by taking cognizance of the fact that a membership function for SCADA control is required. You can now proceed by designing a SCADA rule for controlling of power substation, training these rules in ANN, designing a visual basic model for SCADA control of power substation using PLC and designing a Simulink model for SCADA control of power substation using PLC. The result obtained when compared to the conventional one was 10% better.

Objective: To establish a three-dimensional finite element model of tooth-implant fixed bridge and its supporting tissue and to analyze the stress distribution of bone tissues around the abutments under dynamic load.
Methods: Three dimensional finite-element models were created of two 765 sections of the mandible with tooth and implant independently or tooth-implant fixed bridge embedded in. In a circle of mastication (0.875s), vertical and oblique dynamic loads were applied on the model. The stress distribution was analyzed to study the biomechanical behavior of bone tissue surrounding solely or splinted abutments.
Results: As loading on the models, stress concentration appeared in cervical cortical bone of the abutments and the stress of the bone tissue of the implant is higher than that of the natural tooth. When the lingual load (t=0.26s) on the fixed bridge , the maximum Von mises stress is 141.773 Mpa in the distal cervical of the implant as much as 2.2 times of that tooth, which may reduce disadvantages for implant.
Conclusion: It is feasible to design the restoration of the natural tooth-implant combined supporting double-end fixed bridge, however, various measures should be taken in the design to compensate for the biological differences between them.