Escherichia hermannii is an extremely rare invasive infection aetiological agent, and to this day this strain remains almost unidentified in Morocco.
Our 21-year-old patient was operated on for a closed fracture of the middle third of the right leg and then lost to follow-up.  Eight months later, the patient presented to the trauma department with septic pseudarthrosis of the operated leg that exposed the middle third of the tibia. Following his readmission, specimens containing material were sent to the microbiology laboratory for analysis. Investigations concluded positive in vitro cultures (polymicrobial): pus and bone (Escherichia hermannii, Staphylococcus aureus) and screws (Escherichia hermannii, Pseudomonas aeruginosa).
We report the first identification of the Escherichia hermannii strain in the biology department, Ibn Tofail Hospital, University Hospital Centre (CHU) Mohammed VI of Marrakech.
Gram-negative rods were collected, without spore formation, oxidase negative, catalase positive. A biochemical study on the Api 20E gallery (Bio Mérieux) with data analysis on the UPBM le Lab site "http://lab.upbm.org/identifieur/galerie.php" allowed the identification of Esherichia hermannii with a 98.9% probability of "excellent identification" and the vivid appearance of a yellowish pigment at 48 hours on chocolate agar.
Following the antibiotic susceptibility test, performed by the agar diffusion technique according to the recommendations of the French Society of Microbiology (SFM), all antibiotics tested were sensitive except ampicillin and ticarcillin; and piperacillin "sensitive at high dosage = Intermediate", the patient was put on ciprofloxacin and gentamicin with a favourable evolution.

Background: Urinary tract Infection (UTI) is among the most common infections described in outpatient settings and hospital patients. In almost all cases empirical antimicrobial treatment initiates before the laboratory results of urine culture are available; thus antibiotic resistance may increase in uropathogens due to frequent use of antibiotics.
Material and Method: This retrospective study was conducted for the period of one year i.e from October 2021 to September 2022. Urine samples of Outpatients from the Department of Gynaecology and Obstetrics were sent to the department of Microbiology, Dr, Rajendra Prasad Govt. Medical college and were processed as per the standard guidelines.
Results: Out of these 945 samples only 261(28.6%) urine samples showed pathogenic growth and 684(72.4%) were sterile and showed insignificant growth. In 261 growth positive urine samples 55(21.1%) were gram positive bacteria and 188(72%)were gram negative bacteria and yeast were 18(6.9%). In gram positive bacteria the majority were Staphylococcus aureus 35(63.6%). Amongst gram negative bacteria majority were Escherichia coli 105 (55.9%). Antimicrobial susceptibility showed maximum sensitivity for Fosfomycin 73.9% and Nitrofurantoin 57.9% in gram negative bacteria and similarly for gram positive bacteria with maximum sensitivity for Fosfomycin 72.7% followed by Amoxyclav 67.2%
Discussion & Conclusion: Regular monitoring is required to establish reliable information about susceptibility patterns of urinary pathogens for optimal empirical therapy of patients with UTIs. On the other hand, widespread injudicious use of antibiotics and the subsequent development of antibiotic resistance are growing concerns. Therefore, health care workers should consider regular reviews of antibiograms and choose the appropriate antibiotic in each case.

During the Covid-19 pandemic, antibiotics such as macrolides and fluoroquinolones have experienced increased and irrational prescription.
The aim of this study is to determine the epidemiological profiles of Staphylococcus aureus and antibiotic resistance (January 1 to December 31, 2019) before and after (January 1, 2021 to May 31, 2022) the Covid-19 pandemic at the Avicenne Military Hospital in Marrakech.
Identification and susceptibility testing were performed on Phoenix® M50 to determine MIC, as well as measurement of inhibition diameters by the Mueller-Hinton (MH) agar disc diffusion method.
In the pre-pandemic study period, Staphylococcus aureus accounted for 8.01% of the isolates (n= 1073) and 5.05% of the isolates (1246) during the pandemic.
Staphylococcus aureus isolated (23.25%) in internal medicine followed by the intensive care unit (20.93%) before and after the start of the pandemic the intensive care unit (34.92%) followed by trauma (19.04%). The samples taken were Pus (44.18%) before the start of the pandemic compared to 47.61% after, followed by broncho alveolar lavage fluids (15.11%) compared to 20.63%, then blood cultures 12.79% compared to 11.11%.
Antibiotic resistance increased from 1.16% to 3.17% for Ciprofloxacin; from 2.7% to 4.76% for Levofloxacin, Erythromycinfrom 10.46% to 12.7%; Clindamycinfrom 3.48% to 11.11%.
Due to its high capacity to colonise and persist in the hospital environment, Staphylococcus aureusis important. The inappropriate use of antibiotics in Covid-19 could be the cause of increased resistance.

Information on the constituents of medicinal plants used in Sudanese ethnomedicine could provide a rationale for their traditional uses. Hence this study was designed to investigate the phenolics of the medicinally important species Cordia sinensis which is widely used in ethnomedicine to treat a wide array of human disorders. A Flavone was isolated from the stem bark and its structure was partially elucidated on the basis of its spectral data (UV, 1HNMR and MS).

The 24-hour proteinuria has been considered, for a long time, to be the “gold standard” for the measurement of proteinuria; in order to diagnose and evaluate of the severity of many diseases. However, this assay method presents several constraints, notably those related to the difficulty of collecting the urine sample as well as its transportation to the laboratory, in addition to various physiological variations in protein excretion regarding the state of hydration, physical activity or even the postprandial period. Therefore, the evaluation of proteinuria using the ratio of proteinuria to creatinuria on a urine sample appears more than satisfactory in many situations, as an adequate alternative to the 24-hour proteinuria,
The final aim of our study was to find out if the collection of a urine sample and the calculation of the P/C ratio, could substitute a 24-hour urinary protein samples from kidney disease patients. All the tests were performed at the biochemistry laboratory, using the Alinity automated system (Abbott®).
The overall results show an excellent agreement between the two methods with a kappa coefficient of (0.91). This test, which is simpler, less expensive and has a shorter turnaround time, will also improve the time management of severe pathology, unlike the current situation where the 24-hour proteinuria result is known more than 24 hours after admission.

Schiff bases are the most widely used organic compounds. They have been shown to exhibit a broad range of biological activities, including; antifungal, antibacterial, anti-malarial, anti-proliferative, anti-inflammatory, antiviral and antipyretic properties. In this study the Schiff base ortho- phthalaldehyde semicarbazone was prepared as a new multi-dentate complexing agent, by condensing ortho-phthalaldehyde with semicarbazide hydrochloride in 2:1 molar ratio respectively in ethanolic medium. This ligand was used to synthesize metal complexes of Ni (II) and Co (II) in 1:1 molar ratio using ethanol as a solvent. Characterization and structure elucidation of the ligand and its corresponding metal complexes have been investigated on the basis of molar conductance, UV and IR spectral studies. An in vitro antimicrobial investigation was also carried out for the free ligand and its metal complexes against four bacteria; Bacillus subtitles, Staphylococcus aureus (Gram-positive), Escherichia coli and Pseudomonas aeruginosa (Gram-negative) and one Fungi; Candida albicans, to assess their antimicrobial properties by disc diffusion technique. Antimicrobial activity of the prepared complexes showed higher activity than the free ligand.

Background: Intestinal parasitosis is a public health problem in developing countries. Our study proposes to know the frequency and the favoring factors of intestinal parasitosis in HIV positive patients often very exposed to severe clinical manifestations given their immune status.
Patients and Methods: This is a prospective descriptive study, carried out over a period of 1 year from March 2022 to March 2023, in the laboratory of parasitology and mycology of the HMIMV in Rabat.
Patients with positive HIV serology, with or without digestive signs, were included.
A parasitological examination of the stools was carried out in these patients with, in addition to the standard techniques, the modified Ziehl-Neelsen.
Results and Discussion: 28 patients were included; the average age is 45.4 years with a sex ratio M/F of 6. The rate of parasitic infections is 42.8%, made up exclusively of protozoa, with an overall prevalence of intestinal coccidia at 14.3%. These results are in agreement with certain studies concerning the predominance of protozoa.There was a positive relationship between CD4 count and parasite species. found, our study showed that intestinal coccidiosis being more frequent from a CD4 count of less than 200 elements per mm3, this is in agreement with the literature of which opportunistic intestinal parasitosis are intimately linked to the immune status and in particular the CD4 count.
Conclusion: The intestinal parasitism of our patients is made exclusively of protozoa which are indicators of hygiene.
Hygiene promotion, systematic stool examination or, failing that, deworming of patients will certainly reduce this prevalence.

Small ruminants such as sheep and goats are a common economically low-cost livestock investment for smallholder farmers in Nigeria with several disease limitations affecting livestock productivity, among which is African Animal Trypanosomosis (AAT). In apparently healthy animals, trypanosomosis can best be diagnosed by molecular method compared to the conventional clinical diagnosis and standard trypanosome detection method (STDM). A positive test shows active infection and nucleotide sequences specific for a particular trypanosome species or subspecies. To assess the prevalence of trypanosomes in local breeds of sheep and goats in Bassa and Jos East areas, a total of 400 blood samples collected at random from June to August 2018 were subjected to Polymerase Chain Reaction (PCR) molecular method and Standard Trypanosome Detection Method (STDM) using the microscope for examination [Thin, Thick, Buffy Coat smears and Packed Cell Volume (PCV). The PCV of each animal sampled was assessed. There was no significant difference in the mean PCV between infected Caprine and non-infected Caprine in Bassa and Jos East areas (P>0.05). A significant difference in the mean PCV between infected Ovine and non-infected Ovine in Bassa and Jos East areas (P<0.05) was observed. Results showed 109 (27%) infected with Trypanosoma brucei and 01(0.25%) Trypanosoma vivax by PCR and 0.0% STDM (Thin, Thick, Buffy Coat smears and PCV). The results further showed that small ruminant trypanosomosis even at the sub-clinical level is prevalent in the areas studied.
Towards improved diagnosis and enrichment of the trypanosomosis database, the study recommends the inclusion of molecular diagnostics for more effective planning and implementation of control strategies towards African trypanosomosis elimination in Nigeria.

Acacia nilotica Lam. Family Mimosaceae is known as known as Babool in Hindi. It is a moderate sized tree and found in tropical and subtropical countries. All parts of this plant are used for the treatment of different types of human and animal diseases. The present communication provides a detailed account of the physico-chemical and biochemical study carried out on Babool stem bark. The study includes macroscopy, powder microscopic studies, preliminary phytochemical investigation, physicochemical tests and development of HPTLC (High Performance Thin Layer Chromatography) fingerprints profile. Physicochemical parameters were performed in triplicate and found average values of such as foreign matter 2.63%, loss on drying at 1050C 4.48%, alcohol soluble extractive 36.18%, water  soluble extractive 26.69%, total ash 12.01%, acid in-soluble ash 4.62%. HPTLC (High Performance Thin Layer Chromatography) fingerprints profile of methanolic extract was done by using mobile phase toluene: ethyl acetate (7:3). TLC plate was derivatized by using 5% Methanolic-sulphuric acid derivatizing reagent. Major spots Rf values and colour were recorded at 366nm, after derivatization 366nm and UV light. Florescence studied and biochemical analysis was also done. Various types of phyto-constituents are present such as alkaloids, protein, tannin, resin, saponin and glycosides.  Established parameters can be used as standards for quality control and identification of the plant in herbal compound formulations and also preparation of a monograph of the plant.