Introduction: Leukemia, a malignant monoclonal proliferation of blood-forming cells, can manifest in different ways, including acute lymphoblastic leukemia (ALL) affecting mainly children, and acute myeloid leukemia (AML). The clinical signs of acute leukemia are not always obvious, but clues can alert the doctor. Biological examinations, in particular the hemogram and the blood smear, are crucial for the diagnosis, involving the biologist on the front line to identify, classify and monitor acute leukemias.
Materials and methods: The workshop was set up at the simulation center of the Faculty of Medicine and Pharmacy of Tangier, using audiovisual equipment to simulate the use of an optical microscope in the face of suspected cases of acute myeloid leukemia. The objective was to teach the behavior to adopt when faced with cytological or microscopic particularities, thus facilitating the orientation of the clinical diagnosis and appropriate therapeutic management.
Results: The results showed that the majority of participants improved their skills after the training, with a better ability to distinguish and classify acute myeloid leukemias according to cytological criteria. Before the training, some participants had gaps in distinguishing between AML and ALL, but afterward
Conclusion: The simulation workshop was well accepted, and results indicate that simulation teaching improves learning and acquisition of new knowledge in the field of acute myeloid leukemia.

The field of bioprinting in regenerative medicine is new. The development of three-dimensional structures packed with cells that mimic biological tissues is crucial for medication delivery, cancer research, and tissue engineering. Bioprinting can be used to generate scaffolds for tissue engineering with regulated microstructures, patient-specific spatial geometry, and the arrangement of various cell types. The different manufacturing methods—laser-based, extrusion-based, and inkjet-based bioprinting—are stated, explained, and contrasted in this concise overview. Each technique's principles and examples are addressed along with the state of each technique's research when it comes to different tissue types. Successful bioprinted scaffolds may be manufactured via both nozzle-based (inkjet and extrusion printing) and laser-based (stereolithography and laser-assisted bioprinting) approaches. It has been found that these four methods have various effects on print quality, resolution, and cell survival. It was also discovered that the concentrations and selection of materials influenced the printing properties. Recent study has shown that every method has advantages and disadvantages of its own, and that combined the benefits of different approaches might yield even greater results. An overview of three-dimensional printing's advancements in various medication dosage forms and its uses in the pharma health care systems are given here.

Metallonucleases, which catalyze and repair breaks in DNA strands, were developed because of the requirement for biological control of DNA. They naturally interact with DNA because of their cationic nature, three-dimensional structural profiles, and propensity to carry out hydrolysis, redox, or photoreactions of metal ions and complexes. DNA binding and cleavage are fundamental to cellular transcription and translation, making them a prime target for therapeutic intervention and the creation of structural probes for diagnostic purposes. By inner sphere coordinating with DNA, inorganic compounds like cisplatin and its analogs exhibit anticancer action. Artificial metallonucleases and metal-based chemotherapeutics like cisplatin have been steadily improving over the past few decades. Photo-active octahedral metal complexes have also been effectively employed as light-driven reactive agents and DNA luminescent probes. To enhance their potential as molecular tools for studying genetic material, a current trend involves designing bifunctional assemblies in which a reactive or nucleic acid-recognizing moiety is attached to a photo-active metal centre via a flexible linker. According to this theory, novel metal complexes have been created that either directly conduct redox reactions with DNA or use open coordination sites to facilitate DNA binding and hydrolysis. They can also produce reactive oxygen-containing species or other radicals for DNA oxidation. In addition to cleavage patterns like hydrolytic, oxidative, and photoinduced DNA cleavage, this review briefly discusses drug molecule interaction factors, modes of DNA binding via groove binders, intercalators, and alkylators, and uses the mechanism of cisplatin as an example.

The incidence of periodontal disease in pregnancy and pregnancy induced hypertension are both on the rise. Changes in hormone levels during pregnancy and treatment of pregnancy induced hypertension tend to further aggravate the existing periodontal problems in pregnant women. Therefore, periodontal infection is considered by many scholars to be one of the risk factors for pregnancy induced hypertension. The pathogenesis of both is not yet clear, and it has been found that hypoxia, extracellular matrix degradation, and neutrophil immunomodulation are involved in the pathogenesis. This article aims to investigate the association between periodontal disease in pregnancy and hypertension induced pregnancy and to provide a basis for obstetric care and clinical treatment.

Polyherbal formulations are those that contain two or more herbs in moderation. In Ayurveda, the formulation of drugs is based on two principles: using many drugs and using a single drug. The term "polyherbal formulation" refers to the final. The concept of polyherbalism, while difficult to define in terms of contemporary standards, is unique to Ayurveda. The concept of polyherbalism to achieve increased medicinal efficacy was influenced by the Ayurvedic literature Sarangdhar Samhita. Because polyherbal formulations have so many medical and therapeutic uses, they have been employed all over the world. It is also known as herb-herb combination therapy or polyherbal therapy. Individual plant active phytochemical components are insufficient to achieve the intended therapeutic effects. The utilisation of precise ratios in polyherbal and herbo-mineral combinations can result in heightened therapeutic effects while reducing toxicity. The active ingredients derived from a single plant don't have enough potent pharmacological activity. There is evidence that plant extracts in their crude form are frequently more potent than their constituent parts alone. Rather than using individual chemicals, whole plants or mixes of plants are employed in traditional medicine. Polyherbalism offers some advantages that aren't available in single herbal formulations because of its synergistic effects. With a safe high dose, polyherbal formulations show excellent efficacy in treating a wide range of disorders. There are two ways that synergism functions, depending on the type of interaction: pharmacodynamics and pharmacokinetics. The ability of a herb to facilitate the other herbs' absorption, distribution, metabolism, and elimination is known as pharmacokinetic synergism. On the other side, pharmacodynamic synergism examines the synergistic impact that results from targeting active ingredients with different mechanisms of action but similar therapeutic activity. The important aspects of polyherbal formulation are fully covered in this review.

Any detectable molecular predictor of cancer risk, cancer occurrence, or patient outcome is referred to as a molecular cancer biomarker. Examples of molecular cancer biomarkers include apigenetic signatures, proteomic signatures, and germline or somatic genetic variations. Over the past few decades, great progress has been made in detection technologies. Examples of these advancements include next-generation sequencing, nanotechnology, and the research of exosomes and circulating tumor DNA/RNA. There are several clinical uses for biomarkers. They can be employed as instruments for evaluating cancer risk, screening and early cancer detection, precise diagnosis, prognosis for the patient, forecasting therapeutic response, and cancer surveillance and response monitoring. Given the pivotal role that biomarkers play throughout the whole course of a disease, it is imperative that they go through a thorough review process that includes clinical validation, analytical validation, and an assessment of their clinical utility before being incorporated into standard clinical care. We discuss important milestones in the biomarker development process in this review, along with best practices for avoiding bias introduction and reporting biomarker study results.