Abstract
Purpose: The goal of the study was to investigate the expression of HLA-G, CD16, CD56, CD8, TNF-α , IFN-γ in ectopic pregnancy (EP) by immunohistochemistry.
Methods: The study was conducted on non-pregnant women (NP) (n=10), women with intrauterine pregnancy (IUP) (n=7) and EP (n=10). Immunostaining evaluated by HSCORE analysis of HLA-G, CD16, CD56, CD8, TNF-α, IFN-γ in tubal tissues were compared.
Results: HLA-G showed stronger staining in IUP than other groups (p=0.001). The expression of CD16 was lower in IUP than EP (p=0.005). The expression of CD56 and CD8 was low in NP, while CD8 was highest in EP (p=0.001 and p=0.001). The highest expression for TNF-α was shown in EP, while IFN-γ expression was highest in IUP (p=0.016 and p=0.001, respectively).
Conclusion: The expression of HLA-G from trophoblast in EP is insufficient. The phenotypic characteristics of natural killer cells are different in EP than IUP and the activation of cytotoxic T cell is increased. TNF-α promotes the formation of EP.