Urginea indica Kunth is an important medicinal plant being used extensively to cure innumerable human ailments both by local people and by pharmaceutical companies. It shows great morphological variations and grows in the wild. The development of genetically improved varieties of Urginea indica Kunth which is having such a huge medicinal value would be beneficial in the field of pharmacy. But before the development of genetically improved varieties, the mutagenic screening (detection and verifications of mutagenic activity) of the mutagens on the plants should be done.Therefore, in this research work, the sensitivity of the Urginea indica KunthSynanthus variety to Ethyl Methane Sulphonate was assessed by estimating the frequency of aberrations in the pollen grains of Urginea indica Kunth after treatment for two generations i.e. M1 and M2 generations.The decreased pollen fertility and alteration in the shape and size of the pollen grains with the increasing concentrations of Ethyl Methane Sulphonate in both M1 and M2 generations showed the sensitivity of Urginea indica KunthSynanthus variety to Ethyl Methane Sulphonate.